Discussion: Chabot College Bacteria of The Skin Lab Report

Discussion: Chabot College Bacteria of The Skin Lab Report Discussion: Chabot College Bacteria of The Skin Lab Report The questions in the files. Please see the attached files for more detail Thanks lab_manual_activity__skin_bacteria_lecture.pdf lab_manual_activity__skin_bacteria.pdf lab_activity__3__petrif Chabot College Bacteria of The Skin Lab Report. Lab Manual Activity: Skin Bacteria Submit Assignment Due Monday by 11:59pm Points 10 Submitting a file upload Available until Nov 30 at 11:59pm You were provided a red plate Background reading Pages191-196 in the lab manual Media/Materials MSA (red) petri plate to be stored in the refrigerator Test tube with saline solution 1 sterile swab Procedure Follow the procedure in the lab manual for first and second period. Chabot College Bacteria of The Skin Lab Report. Skip procedure second period #2, the subculture. Skip procedure third period #1, the coagulase test. Label your plates on the bottom side, agar side with the sample. Take several pictures of your plate after you see colonies +/- 2 two days. After you take pictures of your plates, Perform the Catalase test, use hydrogen peroxide. Refer to your lab manual pg. 220, the dichotomous key. It will have the gram stain & catalase reactions. Remember we are attempting to select for the Staphylococcus genus. Please refer to the pictures attached to see samples results. You may skip the Fermentation Test results table on page 196. Sample results: Questions – Answer questions below and upload your responses using the link provided. 1. Why is mannitol salt agar used as a selective medium for normal skin microbiota? 2. List three identifying characteristics of Staphylococcus aureus. 3. List three factors that protect the skin from infection. 4. What is coagulase? How is it related to pathogenicity? 5. Assume that you isolated S. aureus from your skin. How would you determine if it was penicllin-resistant? Lab Activity #3: PetriFilms for Water Sampling Submit Assignment Due Monday by 11:59pm Points 10 Submitting a file upload Available until Nov 30 at 11:59pm Microbes in the Water – Pertifilm Technique IMPORTANT within the hour store the Petrifilms in the refrigerator and check that the bag is properly sealed. The one thing that can ruin the Petrifilm is moisture. By sealing the package, your Petrifilms will stay fresh. Testing Water for E coli and coliforms. You want a water source that should be contaminated with feces or poop. Do not use your toilet as the chlorine will lower any E. coli counts. Do not use ocean samples either, I have tried but there is never any E. coli. You can be creative and think about a water source downstream from where people defecate or poop. Stagnant ponds or sitting water might work. Chabot College Bacteria of The Skin Lab Report. Discussion: Chabot College Bacteria of The Skin Lab Report The samples in the picture were taken downstream from a homeless encampment on the American River. Materials Water source 1 ml per Petifilm Sharpie or ink pen 2 Petrifilms 2 Plastic droppers 4 firm pieces of cardboard cut to 3.25 x 4.25 inches, just large enough to cover the Petrifilm 2 Rubber bands Procedure 1. Label the Petrifilm with the date, time of sample, and where the water source was sampled. 2. Obtain a 1 ml water sample with the plastic dropper 3. Inoculate with the Petrifilm lying on a flat surface, grab the bottom left corner of the film between your thumb and index finger of your left hand. Raise film to expose the red circle of nutrients. Center the pipette with 1 ml vertically over the center of the circle. Squeeze the bulb firmly to expel all the water. Don’t just let go of the film – that often will create bubbles. Slowly lower the film over the water on the nutrient circle until the film is flat over the Petrifilm. 4. Gently lift the inoculated Petrifilm by its edges, and gently tilt the Petrifilm to allow the water to cover the entire circle. 5. After the nutrient circle is covered, let the Petrifilm rest on a flat surface for several minutes before moving. There is a chemical in the nutrients that binds the hydrated nutrients to the film, and this will set in a few minutes. 6. IMPORTANT: When moving the inoculated Petrifilm, NEVER TOUCH THE CENTER AREA – it is a soft gel, and touching will move the gel from the area touched. Move the Petrifilms by holding the edges. 7. Use firm cardboard pieces to hold the Petrifilms while incubating. Take two pieces, label one “Top” and the other “Bottom”, place next to each other with the writing facing down. Collect the Petriflm (or up to 5 Pertrifilms) and stack on the “Bottom” side of one cardboard piece. Place the “Top” cardboard piece on top of the stack, with the writing face up. Secure with a rubber band. You now know that the top of the Petrifilm from the bottom side that has the bottom of the Petrifilm. 8. ORDER NOW FOR CUSTOMIZED AND ORIGINAL NURSING PAPERS In order to get results, the cells of bacteria must grow into colonies of at least 1 million cells. E. coli bacteria grow best at body temperature – they grow in our large intestine, so body incubation is recommended. Body incubation: During the day, place the cardboard with the Petrifilms on the small of your back – between an undershirt and shirt, with a belt holding the package in place. At night you can sleep on top of the Petrifilm pack (“Top” side up). Room temperature: The reason that body temperature is recommended is colonies will appear the quickest at this temperature, between 10-16 hours, whereas at ambient temperature (~20°C, 68°F), colonies will take 24 hours or more to develop. If you choose to use this method, pick a warm spot (i.e., the top of a refrigerator) that will stay warm and check samples after a period of incubation. If there is no growth, keep it there and check back. Results In reading the results, hold the Petriflm by the edges and raise it to eye level. Blue colonies with tiny gas bubbles are the fecal indicator bacterium, Escherichia coli. One to 10 blue E. coli colonies indicate a high disease risk; > 10 E. coli colonies indicates a very high disease risk. Red colonies with gas are bacteria in coliform group that live in the environment – they do not have public health significance in untreated water. The small red colonies with gas are environmental Gram-negative bacteria with no public health significance. Take a picture of your results and submit it for the lab assignment. Sample results Questions – Answer questions below and upload your responses using the link provided. 1. Which of your water samples are potable? 2. What are the differences between E. coli and other coliforms? 3. Why is the Pertifilm useful for scientists and non-scientists working in the field? 4. 5. 6. 7. A water sample may be negative for E. coli and other coliforms and it can still be a public health threat. Why? List five (5) microbes, other than E. coli, that cause infectious diseases associated with drinking water. List the signs and symptoms of E. coli infections and correctly categorize under signs/symptoms. What is the treatment for E. coli infections? Are there any antibiotic resistance issues? … Get a 10 % discount on an order above $ 100 Use the following coupon code : NURSING10

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