Biology

Aseptic Techniques Name ———————–20 ptsObjectives1.- Be aware of where bacteria can be found, and what are the environmental factors that influence the growth of bacteria.2.- Understand the process and importance of labeling and incubating agar plates properly.3.- Learn the aseptic techniques practices necessary to prevent cross-contamination4.- Identify growth of bacteria on the surface of an agar plate, a nutrient broth, and an agar slant.5.- Use colony number estimates to reach conclusions about amount of bacterial growth.6.- Determine the effects of handwashing on bacterial abundance. Determine the effects of bench disinfection on bacterial abundance.7.- Define: pure culture, contamination, aseptic techniques.8.- Know the characteristics of broth medium, bacterial slants, and agar plates.9.- Identify the steps of transferring from broth to broth, slant to slant, and broth to plate.1.- Introduction (4.0 pts.)1.- ( 1 pt.) Explain the following conceptsWhat does the phrase ‘bacteria are ubiquitous’ means?What is media?2.- ( 1pt.) List two locations in a laboratory where bacteria can be found.List two places, in a laboratory, where bacteria should never be found.3.- (1pt.) The first step in working with any type of medium, is to label it properly prior to use.Why are agar plates always labeled at the bottom?Agar plates are always incubated upside down. Explain why.4.- (1 pt.) Incubation temperatures are always given in degree Celsius. The standard incubation temperature of bacteria isolated from human specimens is:Incubating bacteria, isolated from human specimens, at lower temperatures will slow down their growth. Give the approximate temperature (in degree Celsius) of the following incubators:Room temperature incubator:Refrigerator temperature incubator:2.- Ubiquity Exercise (5.0 pts.)In this exercise bacterial presence was investigated in four different environments: banana, soda can, keyboard, and cash.1.- (0.5 pts.) What implement was used to collect the bacterial samples from the 4 different objects?Name the type of medium used in this exercise to grow the bacteria.2.- (1 pt.) Mention two steps that prevented cross-contamination during this exercise.3 .- (1 pt.) During this exercise, what does it mean to ‘streak a plate”?What is a ‘colony’? Are all colonies alike?Where was the cotton tip discarded after each use?Do you think that the medium used to dip the cotton tip was sterile? Explain your answer.4.- (1 pt.) ***Take a picture of the results obtained and upload the picture onto your lab report.4.- (continuation) Complete the table below with the results of the exercise. Note in the chartunder ‘Colony variety’ whether the colonies growing on each plate all look the same, or if there are variations in size, consistency, and color.NUMBER OF COLONIES PER PLATEPLATE LABELAmount of bacteriaColony variety seen/not seen5.- (0.5 pts.) Using the results obtained, explain how handwashing affects bacterial quantity and diversity.6.- (0.5pts.) Using the results obtained, determine which of the 4 objects (soda can, banana, keyboard, and cash) tested had the most amount and variety of bacterial growth. Were the results expected?7.- (0.5pts.) Using the results obtained, explain the effects of ethanol disinfection on the bacterial load and diversity of the lab bench.Is wiping the lab bench with ethanol a method of disinfection, or sterilization? Explain your answer.3.- Aseptic Transfer (3 pts.)1.- ( 1 pt.) The use of aseptic techniques is important in obtaining pure cultures.What are aseptic techniques?What are pure cultures (be specific)?2.- ( 1 pt.) What can happen to a culture if aseptic techniques are not followed?What inoculating tools can be used to transfer microorganisms?3.- (1 pt.) When is the inoculating loop sterilized during a bacterial transfer?When is the mouth of the culture tube passed through the flame during a bacterial transfer?4.- Transfer of E. coli from a broth culture to a sterile broth tube (3 pts.)1.- (1 pts.) Put the steps of bacteria transfer from broth to broth in numerical order. * Note, some steps may be used more than once.

OverviewWhen looking for information about a particular issue, how often do you try to resist biases toward your own point of view?This assignment asks you to engage in this aspect of critical thinking.InstructionsWrite a 3–4 page paper in which you:State your position on the topic you selected in the Week 3 assignment, Conflicting Viewpoints Essay: Part 1. Animal Testing.Identify three premises (reasons) from Animal Testing that support your position, and explain why you selected these specific reasons.Explain your answers to the “believing” questions about the three premises opposing your position from Animal Testing.Examine at least two types of biases that you likely experienced as you evaluated the premises for and against your position.Discuss the effects of your own enculturation or group identification that may have influenced your biases.Discuss whether your thinking about the topic has changed after playing the “Believing Game,” even if your position on the issue has stayed the same.The paper should follow guidelines for clear and organized writing:Include an introductory paragraph and concluding paragraph.Address the main ideas in body paragraphs with a topic sentence and supporting sentences.Adhere to standard rules of English grammar, punctuation, mechanics, and spelling.Use four sources to support your writing. Choose sources that are credible, relevant, and appropriate. Cite each source listed on your source page at least one time within your assignment. For help with research, writing, and citation

1. Many people are confused about the differences between viruses and worms. Why?2. Give one example of the most destructive viruses (Search the Internet) and explain why it is so destructive.

Directions: Answer ALL four of the following questions/prompts in complete sentences and paragraphs. Type your answers directly in the text entry box below. You will be graded on the level of detail you include in your essays and the accuracy of the information you present. This exam is open-book so I expect you to include specific details from the textbook. Do not use direct quotes from the textbook, paraphrase the information you learned from your readings while answering the questions.Textbook: Introduction to Physical Anthropology. 2013-2014/15th edition, Jurmain, Kilgore, Trevathan, & CiochonQuestion 1What does it mean to be a primate? What are characteristics and behaviors all primates have in common? What are characteristics and behaviors that differentiate certain species from one another? What makes humans primates? Provide descriptive analysis of what constitutes mammals of the order primate and include specific examples from the text to support your answer.Question 2How do paleoanthropologists date artifacts and remains? Describe the differences between relative and chronometric dating techniques and provide detailed discussion of one example of each technique in your answer.Question 3What is a hominin and what does it mean to bipedal? Describe the anatomical features necessary for the transition to obligate and habitual bipedalism among our hominin ancestors.Question 4As a species, why is Homo erectussignificant to understandings of our evolutionary past? What are distinctive features and characteristics of Homo erectus skeletal structure and what do we know about their time on Earth?

student was conducting a lab experiment that he conducted a year ago without reading and following the provided instructions. Please discuss the safety guidelines that are not being followed and why? What should the student do instead in order to adhere to the safety guidelines.

In a 1000-word APA essay – Complete all three of the following SherPath case studies: 1. Scope of Pathophysiology, 2. Diagnostic Principles – Gastric Ulcer, and 3. Inflammation and Healing – Fracture and Swelling.Case StudyInflammation and HealingCase Study: Inflammation and Healing – Fracture and Swellingapprox 15 minJL, a 50-year-old woman, fell and broke the left tibia at the ankle. She is in the emergency department, waiting for the fracture to be immobilized. The leg hurts and she notes that the ankle is swelling. A diagnosis of a simple fracture and sprain (damage to ligaments) is made.Question 1What is the cause of pain and swelling? What can JL expect in the days to come as inflammation resolves and healing begins?Answers may vary.

Arthritis is increasingly prevalent among the United States population. Although there are several types of arthritis, the most common (by far) is osteoarthritis. According to the CDC, more than 30 million Americans are diagnosed with osteoarthritis (2018). It’s likely that you or someone you know is struggling with the pain and stiffness associated with osteoarthritis right now.In your discussion post this module, provide at least one prevention technique and one treatment option for osteoarthritis. Describe each item in detail, providing information about how the therapies you chose affect the physiology of the arthritic joints.(please add 2 sources pleases.)

Evolution Research Review TopicScience Daily article + extra research and analysisSources: Use 3 sources:1.Summary of your topic on Science Daily (one ScienceDaily article relating to evolutionary biology), https://www.sciencedaily.com/2.The original paper that is cited by ScienceDaily3. Another paper related to your topic from a scientific journalNOTE: do not pay for access to any article. If you need an article, ask me and I’ll try to get it for you.Review:4 paragraphs (500–800 words) in your own words:Paragraph 1: Summary of the topic you chose combining information from all four sourcesParagraph 2: Significance of the findings.Paragraph 3: Analysis: address these questions:a. Do your sources conflict?b. Do they support/complement one another? How?4. Paragraph 4: Analysis: address these questions:a. What thoughts or questions do you have? especially as it relates to material that was covered in class?

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DescriptionIdentify a current issue your state or our country is dealing with in Health Care. For example: in Arizona and many states this year, Dry Needling has been in the legislative forefront, high co-payments, cuts to the states’ Medicaid program including early intervention is eminent.In your discussion: (Use NY state example)1. What is your issue?2. Who are the stakeholders? 3. What is to be lost or gained? By whom?4. Are you prepared to help in this battle? 5. What is the time frame? 6. Discuss this issue with your colleagues and add comment on their awareness and willingness to help. What can you do to assist?7. If you need ideas contact your State or Federal Government Affairs Chairperson through your state PT Association.